YuanLab
Guangzhou, China中国 · 广州

OUR SCIENCE研究方向

Research areas研究方向

EARLY DEVELOPMENT早期发育CHROMATIN REGULATION染色质调控TRANSCRIPTOME DIVERSITY转录组多样性

01 / EARLY DEVELOPMENT早期发育

Embryos & epigenetic reprogramming早期胚胎与表观遗传重编程

The transition from gametes to an embryo involves extensive changes in gene regulation. Work in this area examines zygotic genome activation and the organization of chromatin during early development.从配子到胚胎的转变伴随着广泛的基因调控变化。相关研究关注合子基因组激活，以及早期发育过程中染色质的组织方式。

Immunostaining of H3K18 crotonylation across mouse oocytes and preimplantation embryos; DAPI labels DNA (blue), H3K18cr is red, and Merge combines the channels. Scale bar: 20 µm.

H3K18 crotonylation in mouse oocytes and early embryos. H3K18cr (red), DNA (blue); scale bar, 20 µm.小鼠卵母细胞与早期胚胎的 H3K18 巴豆酰化。H3K18cr 为红色，DNA 为蓝色；比例尺 20 µm。

Yuan et al. · Advanced Science (2026)Yuan 等 · Advanced Science（2026） · Figure 1d图 1d detail局部 · CC BY 4.0

QUESTIONS & APPROACHES问题与方法

  • When and how does the embryonic genome become active?胚胎基因组何时、如何开始活跃？
  • How do parental contributions and chromatin states relate to early gene regulation?亲本贡献与染色质状态如何关联早期基因调控？

Embryo biology · epigenomics · allele-aware analysis胚胎生物学 · 表观基因组学 · 等位基因分析

Related reading相关论文

ANCHOR: haplotype-aware allelic and isoform inference from single-cell long-read RNA sequencing with de novo variant callingThe Landscape and Regulation of Histone Crotonylation in Mammalian Gametes and Early EmbryosAllelic reprogramming of chromatin states in human early embryos

02 / CHROMATIN REGULATION染色质调控

Meiosis and chromatin regulation减数分裂与染色质调控

Chromatin provides a changing context for genome function. Research in this theme examines histone modifications and their readers, chromatin accessibility, and processes associated with meiotic recombination.染色质为基因组功能提供动态的调控环境。这一主题关注组蛋白修饰及其识别蛋白、染色质可及性，以及减数分裂重组相关过程。

Genome-browser tracks compare DMC1 and ZCWPW1 binding and ATAC-seq chromatin accessibility in wild-type and mutant mouse testes. Orange shading identifies recombination hotspots and blue shading identifies promoter-associated non-hotspot accessibility.

DMC1, ZCWPW1 and ATAC-seq signals in wild-type and mutant mouse testes.野生型及突变小鼠睾丸中的 DMC1、ZCWPW1 与 ATAC-seq 信号。

Yuan et al. · Genome Biology (2022)Yuan 等 · Genome Biology（2022） · Figure 4a图 4a detail局部 · CC BY 4.0

QUESTIONS & APPROACHES问题与方法

  • How do histone modifications and their readers contribute to meiotic processes?组蛋白修饰及其识别蛋白如何参与减数分裂过程？
  • How are chromatin accessibility and DNA repair connected?染色质可及性与 DNA 修复之间有何联系？

Histone regulation · chromatin profiling · integrative analysis组蛋白调控 · 染色质分析 · 多层次整合分析

Related reading相关论文

The Landscape and Regulation of Histone Crotonylation in Mammalian Gametes and Early EmbryosAllelic reprogramming of chromatin states in human early embryosHuman zygotic genome activation is initiated from paternal genome

03 / TRANSCRIPTOME DIVERSITY转录组多样性

Long reads & single-cell transcriptomics长读长与单细胞转录组

A gene can give rise to multiple transcripts. Long-read and single-cell approaches provide complementary ways to investigate that diversity, including isoform and allele-level analyses and studies of ovarian aging.一个基因可以产生多种转录本。长读长与单细胞方法为研究这种多样性提供互补视角，涵盖异构体和等位基因层面的分析，以及卵巢衰老研究。

Complete Figure 1: mouse sample and sequencing workflow, transcript classes, sample correlations, PCA, transcript lengths, exon counts and isoform counts.
Figure 1图 1Wu et al. · Journal of Ovarian Research (2026)Wu 等 · Journal of Ovarian Research（2026） · Source出处 · CC BY-NC-ND 4.0

QUESTIONS & APPROACHES问题与方法

  • What information becomes visible when complete transcripts are resolved?当完整转录本得以解析时，能获得哪些新的信息？
  • How can computational methods connect transcript diversity with biological context?如何用计算方法连接转录本多样性与生物学情境？

Long-read sequencing · single-cell analysis · isoform resolution长读长测序 · 单细胞分析 · 异构体解析

Related reading相关论文

Full-length transcriptomic profiling reveals age-associated isoform remodeling and altered coding potential in the mouse ovaryANCHOR: haplotype-aware allelic and isoform inference from single-cell long-read RNA sequencing with de novo variant calling

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